Sodium Deoxycholate CAS 302-95-4 Assay 97.5~102.5%

Specific Rotation
Take this product, precision weighing, add water to dissolve_quantitative dilution to make about 20mg per lm l solution, according to the law (General 0621), specific rotation of 40.0° to 45.0°.
302-95-4 - Differential diagnosis
Take 10 mg of this product, add 1 drop of sulfuric acid lm l and formaldehyde to dissolve, place for 5 minutes, then add 5ml of water to generate blue-green suspended matter.
The infrared absorption spectrum of this product should be consistent with that of the reference substance of sodium deoxycholate (General Rule 0402).
identification reaction of sodium salt of this product (General Rule 0301).
302-95-4 - Exam
Sodium
Take an appropriate amount of sodium standard solution and dilute it quantitatively with water to make each 1 ml containing 0.16jLtg and 0. 4 Net, 0. 8 ^ tg. 1.0/ig, 1. 2pg solution, as a reference solution; Take this product 0.14g, precision weighing, platinum crucible, slowly heated to complete carbonization, cool, add sulfuric acid 0 .5M l to wet, low temperature heating to sulfuric acid vapor removal after burning at 600℃ to a white gray matter, cool, precision Force 1 into hydrochloric acid lm l dissolved and quantitatively transferred to 100ml measuring flask, diluted with water to the scale, shake, precision take lm l 100ml measuring flask, diluted with water to the scale, as a test solution. The blank solution was prepared by the same method, and was measured and calculated at the wavelength of 589.0 nm by atomic absorption spectrophotometry (General Rule 0406 first method). Based on the dry product, the sodium content should be 5.0%~6.1%.
Clarity and color of solution
Take 0.5g of this product, Add 10ml of water to dissolve, check according to law (General rule 0901 and General Rule 0902 ) , the solution should be clear and colorless. In case of color development, it shall not be deeper than the yellow No. 1 Standard Colorimetric solution (General rule 0901).
Loss on drying
Take this product, at 60℃ under reduced pressure drying to constant weight, weight loss should not exceed 5.0% (General rule 0831).
Heavy metals
take 1.0 g of this product, put it in a platinum crucible, and check it according to law (General rule 0821 second law). The heavy metal content shall not exceed 20 parts per million.
Arsenic salt
Take this product 1.0 g, add 500% magnesium nitrate ethanol solution, light the ethanol, slowly heat to ash, if there is still carbonized, you can add a small amount of nitric acid wet, continue to heat (~ 60(TC)) after complete ashing, cool down, add water 21ml to dissolve, add hydrochloric acid 5ml, check according to law (General rule 0822 first law), should comply with the regulations (0.0002%).
302-95-4 - Content determination
Take this product about 0.3g, precision weighing, add anhydrous formic acid 5 ml to dissolve, add glacial acetic acid 35 ml, potentiometric titration (General Rule 0701), with perchloric acid titration solution (0.1 mol/L) titration, and the results of the titration were corrected with a blank test. Each l of perchloric acid titration solution (0.1 mol/l) corresponds to 41.46mg of C24H39NaO4.