Cholesterol CAS 57-88-5 High Quality

Cholesterol
C27H46O 386.65
Cholest-5-en-3-ol [57-88-5].
Cholesterol is a steroid alcohol used as an emulsifying agent.
Packaging and storage-Preserve in well-closed, light-resistant containers.
Solubility in alcohol-Dissolve 500 mg in 50 mL of warm alcohol in a stoppered flask or cylinder, and allow to stand at room temperature for 2 hours: no deposit or turbidity is formed.
Identification-
A: To a solution of 10 mg in 1 mL of chloroform add 1 mL of sulfuric acid: the chloroform acquires a blood-red color and the sulfuric acid shows a green fluorescence.
B: Dissolve about 5 mg in 2 mL of chloroform, add 1 mL of acetic anhydride, and follow with 1 drop of sulfuric acid: a pink color is produced, and it rapidly changes to red, then to blue, and finally to a brilliant green.
Melting range <741>: between 147° and 150°.
Specific rotation <781S>: between -34° and -38°.
Test solution: 20 mg, undried, per mL, in dioxane.
Acidity-Dissolve 1.0 g in 10 mL of ether in a small flask, add 10.0 mL of 0.10 N sodium hydroxide, and shake for about 1 minute. Heat gently to expel the ether, and then boil for 5 minutes. Cool, dilute with 10 mL of water, add phenolphthalein TS, and titrate with 0.10 N sulfuric acid until the pink color just disappears, stirring the solution vigorously throughout the titration. Perform a blank determination (see Residual Titrations under Titrimetry 541). The difference between the number of mL of 0.10 N sulfuric acid consumed in the blank and the number of mL consumed in the test with the Cholesterol is not more than 0.3 mL.
Loss on drying <731>-Dry it in vacuum at 60 for 4 hours: it loses not more than 0.3% of its weight.
Residue on ignition <281>: not more than 0.1%.