Acetylcholine Chloride CAS 60-31-1 Assay 98.0~102.0%

Acetylcholine Chloride
C7H16ClNO2 181.66
Ethanaminium, 2-(acetyloxy)-N,N,N-trimethyl-, chloride.
Choline acetate (ester) chloride [60-31-1].
Acetylcholine Chloride contains not less than 98.0 percent and not more than 102.0 percent of C7H16 ClNO2, calculated on the dried basis.
Packaging and storage- Preserve in tight container, and store at controlled room temperature.
USP Reference standards <11>-
USP Acetylcholine Chloride RS Click to View Structure
Identification-
A: Infrared Absorption <197K>.
B: To 5 mL of a solution (1 in 10) add 5 mL of silver nitrate TS: a white, curdy precipitate, which is soluble in ammonium hydroxide but insoluble in nitric acid, is formed.
Melting range, Class I <741>: between 149 and 152.
Acidity-Dissolve 100 mg in 10 mL of recently boiled water, and add at once 1 drop of bromothymol blue TS: not more than 0.50 mL of 0.010 N sodium hydroxide is required in order to produce a color change.
Loss on drying <731>-Dry it at 105 for 3 hours: it loses not more than 1.0% of its weight.
Residue on ignition <281>: not more than 0.2%.
Chloride content- Transfer about 280 mg, accurately weighed, to a porcelain casserole, and add 140 mL of water and 1 mL of dichlorofluorescein TS. Mix, and titrate with 0.1 N silver nitrate VS until the silver chloride flocculates and the mixture acquires a faint pink color. Each mL of 0.1 N silver nitrate is equivalent to 3.545 mg of Cl. Not less than 19.3% and not more than 19.8% of Cl, calculated on the dried basis, is found.
Assay-Weigh accurately about 400 mg of Acetylcholine Chloride, and dissolve in 15 mL of water in a glass-stoppered conical flask. Add 40.0 mL of 0.1 N sodium hydroxide VS, and heat on a steam bath for 30 minutes. Insert the stopper, allow to cool, add phenolphthalein TS, and titrate the excess alkali with 0.1 N sulfuric acid VS. Perform a blank determination (see Residual Titrations under Titrimetry 541). Each mL of 0.1 N sodium hydroxide is equivalent to 18.17 mg of C7H16ClNO2.