5-TAMRA CAS 91809-66-4 Purity >97.0% (HPLC at 254nm)

Short Description:

Chemical Name: 5-Carboxytetramethylrhodamine

Synonyms: 5-TAMRA

CAS: 91809-66-4

Purity: >97.0% (HPLC at 254nm) 

Appearance: Red to Dark Purple Powder to Soild

Fluorescent Dye for DNA, Peptides and Proteins

E-Mail: alvin@ruifuchem.com

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Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of 5-Carboxytetramethylrhodamine, 5-TAMRA (CAS: 91809-66-4) with high quality. We can provide COA, worldwide delivery, small and bulk quantities available. Please contact: alvin@ruifuchem.com

Chemical Properties:

Chemical Name 5-Carboxytetramethylrhodamine
Synonyms 5-TAMRA 
CAS Number 91809-66-4
CAT Number RF-PI2188
Stock Status In Stock, Production Scale Up to Tons
Molecular Formula C25H22N2O5   
Molecular Weight 430.45
Spectral Properties Excitation Wavelength: 552 nm
Emission Wavelength: 578 nm
Solubility Soluble In DMSO
Source Synthetic
Category Fluorescent Reagents
Brand Ruifu Chemical


Item Specifications
Appearance Red to Dark Purple Powder to Soild (Visual Inspection)
Identity 1H  NMR, MS Conforms
Purity / Analysis Method >97.0% (HPLC at 254nm)
Column: XDB-C18
Flow Rate: 0.8mL/min
Detection: UV at 254nm
HPLC Elution Buffer: CH3CN:TFA (0.1%)=35:65
Test Standard Enterprise Standard
Shelf Life 12 Months From Manufacture Date if Stored Properly 

Package & Storage:

Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement

Storage Condition: Store in sealed containers at cool and dry place, Freeze (<-15℃), Minimize light exposure, Keep in dark place, Inert atmosphe, Protect from moisture





5-Carboxytetramethylrhodamine, 5-TAMRA (CAS: 91809-66-4), is a long-wavelength dye, fluorescent dye for DNA, peptides and proteins. Amine-reactive. Used for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore. Free 5(6)-TAMRA (ab145282) and 6-TAMRA (ab145338) isomer. 5-TAMRA N-succinimidyl ester (ab145433) precursor. 5-TAMRA, SE and its conjugates yield bright, pH-insensitive orange-red fluorescence with good photostability. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is widely used to label peptides and proteins. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates. Currently AAT Bioquest is using a proprietary process to synthesize isomer-free 5-TAMRA without the conventional chromatographic purification.

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