Fluorescamine CAS 38183-12-9 Purity >98.0% (HPLC)

Short Description:

Chemical Name: Fluorescamine 

CAS: 38183-12-9

Purity: >98.0% (HPLC) 

Appearance: Light Yellow Powder

High Quality, Commercial Production

E-Mail: alvin@ruifuchem.com

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Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of Fluorescamine (CAS: 38183-12-9) with high quality. We can provide COA, worldwide delivery, small and bulk quantities available. Please contact: alvin@ruifuchem.com

Chemical Properties:

Chemical Name Fluorescamine 
Synonyms 4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione 
CAS Number 38183-12-9
CAT Number RF-PI2189
Stock Status In Stock, Production Scale Up to Tons
Molecular Formula C17H10O4 
Molecular Weight 278.26
Stability Hygroscopic
Store Under Inert Gas Store Under Inert Gas
Solubility  Soluble in Acetone, Ethanol, Chloroform, Dimethylsulfoxide and Acetonitrile. Slightly Soluble in Water
Spectral Properties Excitation (nm): 382, Emission (nm): 480, Absorbance (nm): 237
Brand Ruifu Chemical


Item Specifications
Appearance Light Yellow Powder
Purity / Analysis Method >98.0% (HPLC) 
Melting Point  153.0~157.00℃
Water (by Karl Fischer) <0.50%
Total Impurities <2.00%
Carbon by Elemental Analysis 72.88~73.88% (Anhydrous)
Molar Extinction Coefficient Diethyl Ether, 233.0 to 235.0nm
Identity by NMR (CDCl3) Consistent
Solubility in Acetone Colorless to Yellow, Clear, 50.00 mg/ml Pass
Test Standard Enterprise Standard

Package & Storage:

Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement

Storage Condition: Store in sealed containers at cool and dry place; Ventilation; 2~8℃; Protect from light and moisture; Nitrogen protection can extend shelf life





Fluorescamine (CAS: 38183-12-9) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Reaction takes place under mild conditions. Low background due to hydrolysis. Analytical reagent. For fluorescent detection of amino acids, peptides and proteins. Fluorescamine plays an important role as a reagent for the detection of amines, peptides and proteins. It is also used as a substrate for measuring protease activity. It is utilized in the detection and quantitation of residual aminopenicillins and sulfonamides in honey by HPLC. It finds application in the determination of lisinopril in human plasma and urine. Adequately blocks newly generated amino termini in protein sequence analyses. Fluorescamine is a popular amine-fluorogenic reagent for determining protein concentrations in solutions and on gels. It is also used to analyze low molecular weight amines by TLC, HPLC and capillary electrophoresis. Fluorescamine is nonfluorescent but readily reacts with primary aliphatic amines, including those in peptides and proteins, to yield a strongly blue-green fluorescent adduct that can be excited by UV light. The amine adduct has absorption maximum at 385 nm and fluorescence maximum at 486 nm.  

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