PIPES Free Acid CAS 5625-37-6 Purity >99.5% (Titration) Biological Buffer Ultra Pure
Chemical Name | PIPES |
Synonyms | PIPES Free Acid; 1,4-Piperazinediethanesulfonic Acid; 1,4-Piperazinebis(ethanesulfonic Acid); Piperazine-1,4-Bisethanesulfonic Acid; 2,2'-(Piperazine-1,4-diyl)diethanesulfonic Acid |
CAS Number | 5625-37-6 |
Stock Status | In Stock, Production Scale Up to Tons |
Molecular Formula | C8H18N2O6S2 |
Molecular Weight | 302.37 |
Melting Point | >300℃ (lit.) |
Density | 1.4983 |
COA & MSDS | Available |
Sample | Available |
Origin | Shanghai, China |
Product Categories | Biological Buffers |
Brand | Ruifu Chemical |
Item | Specifications |
Grade | Ultra Pure Grade |
Appearance | White Crystalline Powder |
Purity / Analysis Method | >99.5% (Titration) |
Loss on Drying | <0.50% |
Sulphated Ash | <0.10% |
Useful pH Range | 6.1~7.5 |
UV A260nm | ≤0.20 (0.5M in 1N NaOH) |
UV A280nm | ≤0.20 (0.5M in 1N NaOH) |
Solubility | Clear, Colorless Solution (5% 1N NaOH) |
pKa (25°C) | 6.6~7.0 |
Heavy Metals (as Pb) | <5ppm |
Iron (Fe) | <5ppm |
Arsenic (As) | <0.1ppm |
Barium (Ba) | <5ppm |
Calcium (Ca) | <10ppm |
Cadmium (Cd) | <5ppm |
Copper (Cu) | <5ppm |
Iron (Fe) | <5ppm |
Potassium (K) | <50ppm |
DNase | Not Detected |
RNase | Not Detected |
Protease | Not Detected |
Infrared Spectrum | Conforms to Structure |
Test Standard | Enterprise Standard |
Usage | Biological Buffer; Good's Buffer; Pharmaceutical Intermediates |
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Keep the container tightly closed and store in a cool, dry and well-ventilated warehouse away from incompatible substances. Protect from light and moisture. Keep away from fire and heat source.
Shipping: Deliver to worldwide by air, by sea, by FedEx / DHL Express. Provide fast and reliable delivery.


Hazard Symbols Xi - Irritant
Risk Codes 36/37/38 - Irritating to eyes, respiratory system and skin.
Safety Description S22 - Do not breathe dust.
S24/25 - Avoid contact with skin and eyes.
S37/39 - Wear suitable gloves and eye/face protection
S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
WGK Germany 3
TSCA Yes
HS Code 2934999099
PIPES (CAS: 5625-37-6) is used buffering agent in biochemistry, is a Good's buffer notable for having a pKa value similar to ordinary physiological pH. As such, PIPES free acid is frequently used as a buffer in biochemical research. PIPES is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.1~7.5. PIPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. PIPES has wide variety of applications and is commonly used in cell culture media, in protein crystallization, as a running buffer in gel electrophoresis, and as an eluent in isoelectric focusing and chromatography. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay. Solubility of PIPES increases when the free acid is converted to the sodium salt.
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