D-Leucine CAS 328-38-1 H-D-Leu-OH Assay 98.5~101.5% Factory High Quality

pH
Take 0.50g of this product, add 50ml of water, heat to dissolve, cool, and determine according to law (General 0631),pH value should be 5.5~6.5.
Transmittance of Solution
Take 0.50g of this product, add 50ml of water, heat to dissolve, cool, ultraviolet-visible spectrophotometry (General rule 0401), measure the transmittance at 430nm wavelength, not less than 98.0%.
Chloride
Take 0.25g of this product and check it according to law (General rule 0801). Compared with the control solution made of 5.0 ml of standard sodium chloride solution, it should not be more concentrated (0.02%).
Sulfate
Take 1.0g of this product and check it according to law (General rule 0802). Compared with the control solution made of 2.0 ml of standard potassium sulfate solution, it should not be more concentrated (0.02%).
Ammonium Salt
Take this product 0.10g, check according to law (General rule 0808), and compare with the control solution made of standard ammonium chloride solution 2.0 ML, not deeper (0.02%).
Other Amino Acid
Take an appropriate amount of this product, add water to dissolve and dilute to make a solution containing about 20mg per lml as a test solution; Take 1ml for precision measurement and put it in a 200ml measuring flask, dilute to the scale with water, shake, as a control solution; Take the appropriate amount of the leucine reference and the valine reference, and put it in the same measuring flask, water was added to dissolve and diluted to prepare a solution containing about 0.4mg each per 1ml as a system-suitable solution. According to the thin layer chromatography (General 0502) test, absorb the above three solutions each 5 u1, respectively, on the same silica gel G thin layer plate, with n-butanol-water-glacial acetic acid (3:1:1) for development, after deployment, air-drying, spraying with ninhydrin in acetone solution (1-50), heating at 80°C until spots appear, and examination immediately. The control solution should show a clear spot, and the system applicable solution should show two completely separated spots. If the test solution shows impurity spots, the color should not be deeper (0.5%) than the main spot of the control solution.
Loss on Drying
Take this product, dry at 105℃ for 3 hours, loss of weight shall not exceed 0.2% (General rule 0831).
Residue on Ignition
Take 1.0g of this product and check it according to law (General rule 0841). The residue left shall not exceed 0.1%.
Iron Salt
Take 1.5g of this product and check it according to law (General rule 0807). Compared with the control solution made of 0.001% of standard iron solution, it shall not be deeper ().
Heavy Metals
The residue left under the item of taking the ignition residue shall not contain more than 10 parts per million of heavy metal when examined by law (General Principles 0821, Law II).
Arsenic Salt
Add 5ml water, 1ml sulfuric acid and 10ml sulfurous acid, heat to a volume of about 2ml in a water bath, add 5ml water, add Dropwise ammonia solution until the indicator of phenolphthalein is neutral, add hydrochloric acid 5ml, add water to make 28ml, according to the law inspection (General Principles 0822 The first law), should comply with the provisions (0.0001%).
Bacterial Endotoxin
Take this product, check according to law (General 1143), each lg leucine containing endotoxin should be less than 25EU. (For injection)
328-38-1 - Content Determination
Take this product about 0.1g, precision weighing, add anhydrous formic acid 1 ml dissolved, add glacial acetic acid 25ml, according to the potentiometric titration method (General rule 0701), with perchloric acid titration solution (O. 1 mol/L) titration, and the results of the titration were corrected with a blank test. Each 1 ml of perchloric acid titration solution (0.1 mol/L) corresponds to 13.12 mg of C6H13N02.