2,4-Dinitrophenylhydrazine CAS 119-26-6 Purity >98.0% (HPLC)

2,4-Dinitrophenylhydrazine (CAS: 119-26-6), a hydrazine derivative, is a C-nitro compound that is phenylhydrazine substituted at the 2- and 4-positions by nitro groups. It has a role as a reagent. It is a member of phenylhydrazines and a C-nitro compound. And aldehydes, ketone reaction red yellow. It is flammable and toxic. Explosive when dry. Harmful when inhaled, swallowed or in contact with skin. Used as a chromogenic agent for the determination of aldehydes and ketones by photometry and for the determination of aldehydes and ketones by thin layer chromatography. 2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls. It may be used as a colorimetric reagent in the quantitative estimation of prednisone. 

Dinitrophenylhydrazine is relatively sensitive to shock and friction; it is a shock explosive so care must be taken with its use. It is usually supplied wet to reduce its explosive hazard. It is a substituted hydrazine, and is often used to qualitatively test for carbonyl groups associated with aldehydes and ketones. The hydrazone derivatives can also be used as evidence toward the identity of the original compound. Dinitrophenylhydrazine does not react with other carbonyl-containing functional groups such as carboxylic acids, amides, and esters. For carboxylic acids, amides and esters, there is resonance associated stability as a lone-pair of electrons interacts with the p-orbital of the carbonyl carbon resulting in increased delocalization in the molecule. This stability would be lost by addition of a reagent to the carbonyl group.