L-Histidine CAS 71-00-1 (H-His-OH) Assay 98.5~101.0% Factory High Quality
Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of L-Histidine (H-His-OH; L-His; Abbreviated His or H) (CAS: 71-00-1) with high quality. As one of the largest amino acids suppliers in China, Ruifu Chemical manufactures qualified amino acids and derivatives up to international standards, such as AJI, USP, EP, JP and FCC standard. We can provide COA, worldwide delivery, small and bulk quantities available. If you are interested in L-Histidine, Please contact: alvin@ruifuchem.com
| Chemical Name | L-Histidine |
| Synonyms | H-His-OH; L-His; Abbreviated His or H; L-Histidine, Free Base; Laevo-Histidine; L-(+)-Histidin; Histidine; (S)-Histidine; (S)-4-(2-Amino-2-Carboxyethyl)imidazole; (S)-α-Amino-1H-Imidazole-4-Propanoic Acid; (2S)-2-Amino-3-(1H-Imidazol-4-yl)propanoic Acid; 3-(1H-Imidazol-4-yl)-L-Alanine |
| Stock Status | In Stock, Production Capacity 100 Tons per Month |
| CAS Number | 71-00-1 |
| Molecular Formula | C6H9N3O2 |
| Molecular Weight | 155.16 |
| Melting Point | 281℃(dec.)(lit.) |
| Solubility | Soluble in Dilute Hydrochloric Acid |
| Solubility in 1mol/L HCl | Almost Transparency |
| Storage Temp. | Sealed in Dry, Store at Room Temperature |
| COA & MSDS | Available |
| Classification | Amino Acid Derivatives |
| Brand | Ruifu Chemical |
| Hazard Codes | Xn | RTECS | MS3070000 |
| Risk Statements | 22-36/37/38 | F | 10-23 |
| Safety Statements | 24/25-36/37-26-22 | TSCA | Yes |
| WGK Germany | 2 | HS Code | 2933290090 |
| Items | Inspection Standards | Results |
| Appearance | White Crystals or Crystalline Powder, Slightly Bitter Taste | Conforms |
| Identification | Infrared Absorption Spectrum | Conforms |
| Specific Rotation [α]20/D | +12.0° to +12.8° (C=11, 6mol/L HCl) |
+12.3° |
| State of Solution (Transmittance) | Clear and Colorless ≥98.0% | 98.5% |
| Chloride (Cl) | ≤0.020% | <0.020% |
| Sulfate (SO4) | ≤0.020% | <0.020% |
| Ammonium (NH4) | ≤0.020% | <0.020% |
| Iron (Fe) | ≤10ppm | <10ppm |
| Heavy Metals (Pb) | ≤10ppm | <10ppm |
| Arsenic (As2O3) | ≤1.0ppm | <1.0ppm |
| Other Amino Acids | Conforms | Conforms |
| Loss on Drying | ≤0.20% | 0.10% |
| Residue on Ignition (Sulfated) | ≤0.10% | 0.08% |
| Assay | 98.5 to 101.0% (Titration on Dried Basis) | 99.7% |
| pH Test | 7.0 to 8.5 (1.0g in 50ml of H2O) | 7.5 |
| Origin | Non-Animal Source | Conforms |
| Conclusion | Accords with the Standard of AJI97; USP 40 | |
| Shelf Life | Shelf Life 24 Months From Manufacture Date if Stored Properly | |
| Main Uses | Food Additives; Pharmaceuticals; Nutritional Supplements | |
L-Histidine (H-His-OH) (CAS: 71-00-1) AJI97 Test Method
Identification: Compare the infrared absorption spectrum of the sample with that of the standard by potassium bromide disc method.
Specific Rotation [α]20/D: Dried Sample, C=11, 6MOL/L HCl
State of Solution (Transmittance): .5g in 10ml of H2O, dissolve by heating spectrophotometer, 430nm, 10nm cell thickness.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml of 0.01mol/L HCl
Sulfate (SO4): 1.2g, (1), ref: 0.50ml of 0.005mol/L H2SO4
Iron (Fe): 1.5g, (1), ref: 1.5ml of Iron Std. (0.01mg/ml)
Heavy Metals (Pb): 2.0g, (1), pH=7, ref: 2.0ml of Pb Std. (0.01mg/ml)
Arsenic (As2O3): 2.0g, (1), ref: 2.0ml of As2O3 Std.
Other Amino Acids: Test Sample: 50μg, B-6-a, control: L-His 0.25μg
Loss on Drying: at 105℃ for 3 hours.
Assay: Dried sample, 150mg, (1), 2ml of formic acid, 50ml of glacial acetic acid, 0.1mol/L HCLO4 1ml=15.516mg C6H9N3O2
pH Test: 1.0g in 50ml of H2O)
L-Histidine (H-His-OH) (CAS: 71-00-1) USP36-NF31 Test Method
DEFINITION
Histidine contains NLT 98.5% and NMT 101.5% of L-Histidine (C6H9N3O2), calculated on the dried basis.
IDENTIFICATION
A. INFRARED ABSORPTION <197K> The Sample and USP L-Histidine RS are previously recrystallized from 80% alcohol.
ASSAY
PROCEDURE
Sample: 150 mg of Histidine
Blank: Mix 3 mL of formic acid and 50 mL of glacial acetic acid.
Titrimetric system
(See Titrimetry <541>)
Mode: Direct titration
Titrant: 0.1 N perchloric acid VS
Endpoint detection: Potentiometric
Analysis: Dissolve the Sample in 3 mL of formic acid and 50mL of glacial acetic acid. Titrate very slowly with the Titrant. Perform the Blank determination.
Calculate the percentage of Histidine (C 6H9N3O2) in the Sample taken:
Result = {[(VS-VB) x N x F]/W} x100
VS= Titrant volume consumed by the Sample (mL)
VB= Titrant volume consumed by the Blank (mL)
N= actual normality of the Titrant (mEq/mL)
F= equivalency factor, 155.2 mg/mEq
W= Sample weight (mg)
Acceptance criteria: 98.5%~101.5% on the dried basis
IMPURITIES
RESIDUEON ON IGNITION <281>: NMT 0.4%
CHLORIDE AND SULFATE, Chloride <221>
Standard solution: 0.50mL of 0.020 N hydrochloric acid
Sample: 0.73g of Histidine
Acceptance criteria: NMT 0.05%
CHLORIDE AND SULFATE, Sulfate <221>
Standard solution: 0.10mL of 0.020 N sulfuric acid
Sample: 0.33g of Histidine
Acceptance criteria: NMT 0.03%
IRON <241>: NMT 30ppm
Delete the following:
HEAVY METALS, Method I <231>: NMT 15ppm
RELATED COMPOUNDS
System suitability solution: 0.4mg/mL each of USP L-Histidine RS and USP L-Proline RS
Standard solution: 0.05mg/mL of USP L-Histidine RS in water. [NOTE-This solution has a concentration equivalent to 0.5% of the Sample solution.]
Sample solution: 10mg/mL of Histidine in water
Chromatographic system (See Chromatography <621>, Thin-Layer Chromatography.)
Mode: TLC
Adsorbent: 0.25-mm layer of chromatographic silica gel mixture
Application volume: 5μL
Developing solvent system: Butyl alcohol, glacial acetic acid, and water (3:1:1)
Spray reagent: 2 mg/mL of ninhydrin in a mixture of butyl alcohol and 2N acetic acid (95:5)
System suitability
Suitability requirements: The chromatogram of the System suitability solution exhibits two clearly separated spots.
Analysis
Samples: System suitability solution, Standard solution, and Sample solution.
After air-drying the plate, spray with Spray reagent, and heat between 100° and 105° for 15 min. Examine the plate under white light.
Acceptance criteria: Any secondary spot of the Sample solution is not larger or more intense than the principal spot of the Standard solution.
Individual impurities: NMT 0.5%
Total impurities: NMT 2.0%
SPECIFIC TESTS
OPTICAL ROTATION, Specific Rotation <781S>
Sample solution: 110 mg/mL in 6 N hydrochloric acid
Acceptance criteria: +12.6° to +14.0°
PH <791>
Sample solution: 20 mg/mL solution
Acceptance criteria: 7.0~8.5
LOSSON ON DRYING <731>: Dry a sample at 105℃ for 3 h: it loses NMT 0.2% of its weight.
ADDITIONAL REQUIREMENTS
PACKAGING AND STORAGE: Preserve in well-closed containers.
USP REFERENCE STANDARDS <11>
USP L-Histidine RS
USP L-Proline RS
Package: Fluorinated Bottle, 25kg/bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Store in sealed containers at cool, dry and ventilated warehouse away from incompatible substances. Protect from light and moisture.
How to Purchase? Please contact Dr. Alvin Huang: sales@ruifuchem.com or alvin@ruifuchem.com
15 Years Experience? We have more than 15 years of experience in the manufacture and export of a wide range of high quality pharmaceutical intermediates or fine chemicals.
Main Markets? Sell to domestic market, North America, Europe, India, Korea, Japanese, Australia, etc.
Advantages? Superior quality, affordable price, professional services and technical support, fast delivery.
Quality Assurance? Strict quality control system. Professional equipment for analysis include NMR, LC-MS, GC, HPLC, ICP-MS, UV, IR, OR, K.F, ROI, LOD, MP, Clarity, Solubility, Microbial limit test, etc.
Samples? Most products provide free samples for quality evaluation, shipping cost should be paid by customers.
Factory Audit? Factory audit welcome. Please make an appointment in advance.
MOQ? No MOQ. Small order is acceptable.
Delivery Time? If within stock, three days delivery guaranteed.
Transportation? By Express (FedEx, DHL), by Air, by Sea.
Documents? After sales service: COA, MOA, ROS, MSDS, etc. can be provided.
Custom Synthesis? Can provide custom synthesis services to best fit your research needs.
Payment Terms? Proforma invoice will be sent first after confirmation of order, enclosed our bank information. Payment by T/T (Telex Transfer), PayPal, Western Union, etc.
L-Histidine (H-His-OH) (CAS: 71-00-1) Product Function
1. L-Histidine is an essential amino acid that cannot be formed by other nutrients, and must be in the diet to be available to the body.
2. Most often recognized as a precursor to the allergy symptom producing hormone histamine, both histidine and histamine have essential roles in the body beyond tormenting allergy sufferers.
3. Histamine is well known for its role in stimulating the inflammatory response of skin and mucous membranes such as those found in the nose - this action is essential in the protection of these barriers during infection.
4. Histamine also stimulates the secretion of the digestive enzyme gastrin. Without adequate histamine production healthy digestion can become impaired. Without adequate L-histidine stores, the body cannot maintain adequate histamine levels.
5. Less well known is that L-histidine is required by the body to regulate and utilize essential trace minerals such as copper, zinc, iron, manganese and molybdenum
L-Histidine (H-His-OH) (CAS: 71-00-1) Uses
1. Nutritional supplements. Amino acid infusion and comprehensive amino acid preparation are very important components. Medically, it is used to treat gastric ulcer, anemia, allergy, etc. It is used for biochemical research and medicine for the treatment of gastric ulcer, anemia, allergy, etc.
2. Amino acid drugs. The main components of amino acid infusion and comprehensive amino acid preparation are used to treat cardiovascular diseases such as gastric ulcer, anemia, angina pectoris, arteritis and cardiac insufficiency.
3. It is an important component of amino acid infusion and compound amino acid preparation. It can be used to treat gastric ulcer. It is also used in biochemical research.
4. Used as pharmaceutical raw materials and food additives.
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