2′,7′-Dichlorofluorescein Diacetate CAS 2044-85-1 (DCFDA) Purity >98.0% (HPLC)

Short Description:

Chemical Name: 2′,7′-Dichlorofluorescein Diacetate

Synonyms: Diacetyldichlorofluorescein; DCFDA 

CAS: 2044-85-1

Purity: >98.0% (HPLC)

Appearance: White to Off-White Powder or Crystals

Contact: Dr. Alvin Huang

Mobile/Wechat/WhatsApp: +86-15026746401

E-Mail: alvin@ruifuchem.com


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Description:

Ruifu Chemical is the leading manufacturer of 2′,7′-Dichlorofluorescein Diacetate (DCFDA) (CAS: 2044-85-1) with high quality, suitable for fluorescence. Ruifu Chemical can provide worldwide delivery, competitive price, small and bulk quantities available. Purchase 2′,7′-Dichlorofluorescein Diacetate, Please contact: alvin@ruifuchem.com

Chemical Properties:

Chemical Name 2′,7′-Dichlorofluorescein Diacetate 
Synonyms Diacetyldichlorofluorescein; DCFDA; DCFHDA; Dcfh-DA; DCFH2-DA; 2',7'-Dichlorofluorescein 3',6'-Diacetate; 2',7'-Dichloro-3-oxo-3H-Spiro[isobenzofuran-1,9'-xanthene]-3',6'-Diyl Diacetate
Stock Status In Stock, Commercial Production  
CAS Number 2044-85-1
Molecular Formula C24H14Cl2O7
Molecular Weight 485.27 g/mol
Melting Point 232.0~234.0℃(lit.) 
Density 1.59±0.10 g/cm3 
Solubility  Soluble in DMSO 
COA & MSDS Available
Origin Shanghai, China
Product Categories   Fluorescein and Derivatives; Fluorescent Labels; Fluorescent Probes  
Brand Ruifu Chemical

Specifications:

Items Inspection Standards Results
Appearance White to Off-White Powder or Crystals Complies      
Purity / Analysis Method >98.0% (HPLC)  Complies
Residual Solvent  ≤10.0% of Acetic Acid Complies
Infrared Spectrum Consistent with Structure  Complies      
1 H NMR Spectrum Consistent with Structure Complies      
Conclusion The product has been tested & complies with the specifications

Package/Storage/Shipping:

Package: Bottle, Aluminium foil bag, or according to customer's requirement.
Storage Condition: Keep the container tightly closed and store in a cool, dry (2~8℃) and well-ventilated warehouse away from incompatible substances. Protect from light and moisture.
Shipping: Deliver to worldwide by air, by FedEx / DHL Express. Provide fast and reliable delivery. 

HPLC Test Method:

HPLC
Column: Agilent XDB-C18 150*4.6mm,5um
Solvent: acetonitrile : water=70 : 30 0.5% acetic acid
Flow: 1mL/min
Column Temperature: 35℃
Wavelength: 254nm
Injection Volume: 0.005mL
Sample Solution: mobile phase

Advantages:

Sufficient Capacity: Sufficient facilities and technicians

Professional Service: One stop purchasing service

OEM Package: Custom package and label available

Fast Delivery: If within stock, three days delivery guaranteed

Stable Supply: Maintain reasonable stock    

Technical Support: Technology solution available

Custom Synthesis Service: Ranged from grams to kilos

High Quality: Established a complete quality assurance system

FAQ:

How to Purchase? Please contact Dr. Alvin Huang: sales@ruifuchem.com or alvin@ruifuchem.com 
15 Years Experience? We have more than 15 years of experience in the manufacture and export of a wide range of high quality pharmaceutical intermediates or fine chemicals.
Main Markets? Sell to domestic market, North America, Europe, India, Korea, Japanese, Australia, etc.
Advantages? Superior quality, affordable price, professional services and technical support, fast delivery.
Quality AssuranceStrict quality control system. Professional equipment for analysis include NMR, LC-MS, GC, HPLC, ICP-MS, UV, IR, OR, K.F, ROI, LOD, MP, Clarity, Solubility, Microbial limit test, etc.
SamplesMost products provide free samples for quality evaluation, shipping cost should be paid by customers.
Factory AuditFactory audit welcome. Please make an appointment in advance.
MOQ? No MOQ. Small order is acceptable.
Delivery Time? If within stock, three days delivery guaranteed.
TransportationBy Express (FedEx, DHL), by Air, by Sea.
Documents? After sales service: COA, MOA, ROS, MSDS, etc. can be provided.
Custom SynthesisCan provide custom synthesis services to best fit your research needs.
Payment TermsProforma invoice will be sent first after confirmation of order, enclosed our bank information. Payment by T/T (Telex Transfer), PayPal, Western Union, etc. 

2044-85-1 - Safety Information:

Hazard Symbols Xi - Irritant
Risk Codes
R36/37/38 - Irritating to eyes, respiratory system and skin.
R34 - Causes burns
R20/22 - Harmful by inhalation and if swallowed.
Safety Description
S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S36 - Wear suitable protective clothing.
S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.)
S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection.
UN IDs 1759
WGK Germany 3
FLUKA BRAND F CODES 3-8-10

2044-85-1 - Application:

2′,7′-Dichlorofluorescein Diacetate (DCFDA) (CAS: 2044-85-1), BioReagent, suitable for fluorescence. It is the stable storage form of dichlorofluorescein.
Uses can be used as an excellent gas phase substitute for underivatized fluorescein, and as a component / substrate for the determination of 2′,7′-Dichlorofluorescein Diacetate to quantify reactive oxygen species (ROS).
2′,7′-Dichlorofluorescein Diacetate is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-Dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.  
2′,7′-Dichlorofluorescein Diacetate is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress.

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